PALM CombiSystem 通过使用光学捕获和微光束技术分离细胞、细胞团及生物分子。

PALM CombiSystem

在单个系统中运用激光显微切割和光镊技术

PALM CombiSystem

PALM CombiSystem 将两种非接触激光技术融为一体。您可精准地进行显微切割操作并捕获感兴趣的样品,以及使用光陷阱捕获诸如活体细胞等的小尺寸样品。

  • 介绍
    PALM CombiSystem 结合了激光显微切割技术与光学捕获技术

    显微操作既精准又简便

    PALM CombiSystem 结合了激光显微切割技术与光学捕获技术。进行激光显微切割时,先在屏幕上标记一个目标,再启用激光将其从不需要的组织中分离出来。靶向激光脉冲会无接触地将目标向上‘弹射’至一个用于后续分析的收集容器内。

    PALM CombiSystem 的光陷阱能以非常高精度操作细胞和亚细胞级颗粒。您可以使用光的学力量直观地捕获、移动和定位显微样品,如血红细胞和细菌。

     即刻联系蔡司了解更多有关 PALM CombiSystem 的信息!

  • 特点
    PALM CombiSystem 结合了激光显微切割技术与光学捕获技术

    将系统扩展成一个完整的研究级平台

    PALM CombiSystem 能出色地控制激光显微切割操作。它可以实现:

    • 显微切割样品,用于DNA、RNA 和蛋白质分离的固定样品以及活细胞
    • 可应用于冰冻切片、FFPE(福尔马林固定石蜡包埋)组织切片、细胞涂片、细胞离心涂片、染色体制备及活细胞
    • 以非常高的精度捕获、分选和定位
    • 操作和移动液体内的颗粒及活细胞
    • 连接模块化设备,例如:用于荧光和明场的 AxioCam 高分辨率数码相机,让成像更清晰
    • 直观的用户接口和功能,使其易于集成至工作流程中从蔡司专家的专业知识与技术支持服务中受益
    • 从蔡司专家的专业知识与技术支持服务中受益


    蔡司研制的 PALM CombiSystem 是一套可集成面向未来技术的平台 — 即使在实验室内使用多年也不会落伍。

     即刻联系蔡司了解更多有关 PALM CombiSystem 系统及使用其光镊和激光显微切割系统的信息!

  • 集成性
    PALM CombiSystem 结合了激光显微切割技术与光学捕获技术

    PALM CombiSystem 与蔡司系统组合应用

    PALM CombiSystem 与 Axio Observer 倒置式研究级显微镜可以配套提供。这就意味着您不仅能从光学捕获和显微切割性能中受益,而且还可获得蔡司超凡的成像品质。

    此外,PALM CombiSystem 与许多其他的蔡司组件兼容,可用于您的系统扩展及搭建多功能研究级显微平台,让您的投资得到更大回报。

     即刻联系蔡司了解更多有关 PALM CombiSystem 系统及使用其光镊和激光显微切割系统的信息!

  • 技术

    无接触的高精度显微操作

    PALM CombiSystem 使用聚焦激光束无接触地切割和分离所选样本。专利型激光压力弹射装置能快速且无污染地分离目标样品。

    此外,PALM CombiSystem 还提供光镊技术。如果需要一款用于分离和操作细胞与亚细胞级颗粒的高精密工具,那么它必将是您的理想之选。

     即刻联系蔡司了解更多有关 PALM CombiSystem 系统及使用其光镊和激光显微切割系统的信息!

     

  • 应用

     即刻联系蔡司公司了解更多有关 PALM CombiSystem 系统及使用其光镊和激光显微切割系统的信息!

  • 下载

    PALM Family - A New Dimension in Sample Purity

    Integrated Laser Microdissection and Microscope Systems for Live Cells and Fixed Material

    页: 23
    文件大小: 4.842 kB

    Application Note: Laser Microdissection: A Key Technology for Microproteomics

    Isolate a single cell population Isolate 30.000 cells in one step within seconds Seamless integrate LCM into your workflow

    页: 7
    文件大小: 2.198 kB

    On-Chip Single Cell Analysis - for PALM MicroBeam

    For Single Cell Genotyping, Sequencing and Expression Analysis: Reliable, Safe, Reproducible

    页: 2
    文件大小: 2.117 kB

    Publications

    Including MicroBeam, CombiSystem & MicroTweezers
    Download

    Protocols of ZEISS Labs

    The Unique Microdissection Service

    LCM Protocols – Protein Handling for LC/MS
    Non contact Laser Capture Microdissection - June 2011
    (24 pages)
    Download (7.630 kB)

    PALM Protocols – RNA handling
    Dos and Don’ts, slide preparation, archived samples, staining procedures, storage, laser microdissection procedure, collection devices and procedures. Downstream applications, RNA from FFPE and frozen sections, QIAGEN RNeasy FFPE kit, quality control, lab tips.
    (28 pages)
    Download (612 kB)

    PALM Protocols – DNA handling
    Preparation and treatment of slides, mounting samples onto slides, staining procedures, laser microdissection procedure, collection devices and procedures, cap check. Downstream applications, DNA from FFPE and frozen sections. QIAamp DNA procedure. PCR set up.
    (26 pages)
    Download (8.017 kB)

    Chromosome Preparation
    Chromosome preparation, staining, procedure of laser microdissection, collection procedures, collection onto AmpliGrid slide, consumables and components. Collection devices, lenses.
    (20 pages)
    Download (7.147 kB)

    Cell Culture, non-contact Laser Capture Microdissection (LCM) and Recultivation
    Cultivation of cells, coating of Dishes and Slides, improvement of visualization, procedure of laser microdissection, recultivation, time lapse, incubators. Optical Tweezers.
    (14 pages)
    Download (500 kB)

    Immunofluorescence on frozen sections
    Tips before starting, microtome cutting, antibody staining.
    (5 pages)
    Download (437 kB)

    FISH Hybridization
    Chromosome preparation. Preparation of chromosome paints. Hybridization of painting probes. Preparation of centromeric probes.
    (15 pages)
    Download (389 kB)

    AutoLPC from glass slides results in good quality RNA
    For RNA experiments on microdissected samples we usually recommend the use of MembraneSlides. Sometimes only old archived slides are available. We show that even this kind of slides is useful for RNA preparation. After removing the coverslip the unique and proprietary software function of “AutoLPC” allows quick access to the desired tissue area. And we could prove that this method does not influence the RNA quality.
    (6 pages)
    Download (2.287 kB)

    RNA extraction from FFPE sections
    Short protocol. Tips and recommendations for using FFPE sections as source for RNA extraction.
    (4 pages)
    Download (568 kB)

    RNA extraction from frozen sections
    Short protocol. Especially helpful for RNase rich tissue. Tips for microtome cutting and special staining (cresylviolet).
    (4 pages)
    Download (179 kB)