Origin of Fluorescence

Under irradiation with light of a wavelength lex, certain electrons of a fluorochrome are raised to a higher energy level. During a very short dwell time, they lose some of their energy and drop back to their original level while emitting light of a longer wavelength lem>lex. The difference in wavelengths is known as the Stokes shift.
In multiphoton excitation, the energies of several photons with n times the excitation wavelength add together to raise the electrons to the higher energy level.

Know How

Confocal Principle

Origin of Fluorescence

Fluorescence Spectra

Emission Separation

Energy diagram of fluorescence excitation.
Single photon excitation (left), multiphoton excitation (right)

	Origin of Fluorescence

	Under irradiation with light of a wavelength lex, certain electrons of a fluorochrome are raised to a higher energy level. During a very short dwell time, they lose some of their energy and drop back to their original level while emitting light of a longer wavelength lem>lex. The difference in wavelengths is known as the Stokes shift. In multiphoton excitation, the energies of several photons with n times the excitation wavelength add together to raise the electrons to the higher energy level.		Know How Confocal Principle Origin of Fluorescence Fluorescence Spectra Emission Separation


	Energy diagram of fluorescence excitation. Single photon excitation (left), multiphoton excitation (right)